CRISPR utility
PegRNA Checker
Lightweight QC for PBS and RT template blocks. No planner calls—just quick heuristics to flag issues.
PBS
Primer binding site
Length
— nt
GC%
—%
Tm (Wallace)
—
PBS short (<8 nt)PBS GC is low
RT template
Reverse transcript template
Length
— nt
GC%
—%
Tm (Wallace)
—
RT template short (<10 nt)RT GC is low
Verdict
Add both PBS and RT to score the pegRNA.